Mediators of allergy, such as leukotrienes or histamine are produced and/or secreted upon contact of allergen with IgE molecules on specialized cells such as basophils and mast cells. In contrast to histamine, leukotrienes are also synthesized and released in the case of the pseudo-allergies, reactions resembling allergy, but without involvement of any IgE molecules.

CAST^®-2000 the Cellular Antigen Stimulation Test, can also be considered as an in vitro allergy provocation test. The collected patient's leukocyte blood fraction will be exposed in vitro to the suspected specific allergen. After incucation the cellular supernatant will be tested for the de novo synthesis of sulfidoleukotrienes by a highly specific ELISA.

This test enables not only the detection of IgE-mediated allergies but also the pseudo-allergies. This latter kind of allergy is best known for Aspirin (ASA) and other non steroidal anti-inflammatory drugs (NSAIDs) and various other drugs or food additives.

Product Information

CAST®-2000
Method	ELISA
Time to results	5.5 h
Sample Type	200 µl whole EDTA blood
Standard Range	50-3200 pg/ml
Sensitivity	19 pg/ml
Order Code	EK-CAST 192 wells EK-CAST5 480 wells
	CE

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Main applications for the CAST^®-2000

The CAST^® assays can be used for the confirmation of any IgE mediated allergy with controversial results from classical testing (anamnese, skin testing, spec. IgE). Further information about specific fields of allergy ask for independent testing on Cellular Allergy for human and equine application.